OXIDANT EFFECTS ON CULTURED HUMAN NEURAL CELLS. † 1706

Oxidant-induced apoptosis in cultured human retinal pigment epithelial cells.

PURPOSE To determine the mechanism of oxidant-induced cell death in cultured human retinal pigment epithelium (hRPE). METHODS Cultured hRPE cells were treated with different concentrations of a chemical oxidant, t-butylhydroperoxide (tBH), for different periods of time. Apoptosis was determined with terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick-end labeling (TUNEL) and flow c...

The Effects of Lithium Chloride on Survival and Function of in Vitro Cultured Human Granulosa Cells

Purpose: To investigate the effect of lithium chloride (LiCl) on survival and functional activity of in vitro cultured human granulosa cells (GCs). Materials and Methods: GCs were isolated from follicular fluid of women undergoing IVF/ICSI. After RBCs removal, GCs were plated in a medium containing varying concentrations of LiCl including 1, 2 and 5mM for two weeks period. A culture without Li...

Effects of p-Nitrotoluene on Cultured Mesencephalic Neural Stem Cells

Current risk assessment methods for environmental chemicals are based on adult physiology. However, recent reports have shown an increased incidence of neurodevelopmental disorders which may result from exposure to chemical in utero and during the early postnatal period. We previously showed that exposure of neonates to the environmental chemical p-nitrotoluene caused hyperactivity, accompanied...

Anti-oxidant and Selective Anti-proliferative Effects of the Total Cornicabra Olive Polyphenols on Human Gastric MKN45 Cells

Background: According to the epidemiological studies, consuming olive products can decrease the incidence of the different types of cancers mostly due to the high anti-oxidant properties of their polyphenolic compounds. Objectives: To evaluate the anti-oxidant and anti-proliferative potentials of the olive fruits total polyphenols on the gastric adenocarc...

Effects of bioflavonoids on cultured human retinal pigment epithelial cells